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Books > Science & Mathematics > Biology, life sciences > Biochemistry > Proteins
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Multiprotein Complexes - Methods and Protocols (Paperback, 1st ed. 2021)
Loot Price: R3,875
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Multiprotein Complexes - Methods and Protocols (Paperback, 1st ed. 2021)
Series: Methods in Molecular Biology, 2247
Expected to ship within 10 - 15 working days
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This volume explores strategies and detailed protocols for the
preparation of macromolecular complexes and their characterization
in view of structural analysis. The chapters in this book are
separated into three parts: Part One focuses on sample preparation,
and covers strategies for recombinant expression of multiprotein
complexes in prokaryotic and eukaryotic hosts, for genome
engineering using the CRISPR/Cas9 system and for production of
specific binders such as reformatted antibodies and artificial
binding proteins. Part Two looks at the biophysical methods that
can provide useful indicators for sample optimization, and often
complement structural information obtained with core technologies
for structure determination-x-ray crystallography and cryo-electron
microscopy-by quantitative solution data. Part Three discusses the
characterization of multiprotein complexes in a cellular
environment using the latest technologies and in vivo approaches.
Written in the highly successful Methods in Molecular Biology
series format, chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Cutting-edge and
authoritative, Multiprotein Complexes: Methods and Protocols is a
valuable resource for structural and molecular biologists who need
to prepare multi-components for their applications, and for other
scientists working on macromolecular assemblies from other angles
that need to know the latest approaches that the field has to
offer.
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