Two decades have passed since the mechanisms of protein synthesis
became well enough understood to permit the genetic modification
oforganisms. An impressive amount of new knowledge has emerged from
the new technology, but much ofthe promise of20years ago has notyet
been fulfilled. In biotechnology, efforts to increase the yields of
commercially valuable metabolites have been less successful than ex
pected, and when they have succeeded it has often been as much from
selective breeding as from new methods. The cell is more
complicated than what is presented in the classical teaching of
biochemistry, it contains more structure than was dreamed of 20
years ago, and the behaviour ofany systemofenzymes is more
elaborate than can be explained in terms ofa single supposedly
rate-limiting enzyme. Even if classical enzymology and meta bolism
may have seemed rather unfashionable during the rise ofmolecular
biology, they remain central to any modification ofthe metabolic
behaviour oforganisms. As such modification is essential in much
ofbiotechnology and drug development, bio technologists can only
ignore these topics at their peril."
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