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Molecular Ecology and Evolution: Approaches and Applications (Paperback, Softcover reprint of the original 1st ed. 1994)
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Molecular Ecology and Evolution: Approaches and Applications (Paperback, Softcover reprint of the original 1st ed. 1994)
Series: Experientia Supplementum, 69
Expected to ship within 10 - 15 working days
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In the last 25 years, we have witnessed a revolution in the way
that ecologists and evolutionary biologists approach their
disciplines. This revolution has been fueled by the ability to
dissect the genetic and molecular basis of variation that is partly
the currency of these disci- plines. Using modern molecular
techniques, we have begun to restruc- ture the spectrum of
questions that can be addressed in studying the mechanisms and
consequences of the ecology and evolution of living organisms. The
molecular revolution has co me in waves, so to speak, with three
particularly important developments. The first concerns the
establish- ment and widespread use of pro tein analysis.
Microcomplement fixation and isozyme e1ectrophoresis were the
techniques around which much of the genetic work in ecology and
evolution were once based. The next wave started with the
development of recombinant DNA technology and centered around the
use of restriction fragment length polymor- phisms (RFLPs) and
sequencing of DNAs cloned in bacteria. This technology was the
first to actually examine and accumulate genetic information at the
nucleotide level. The most recent wave of technology that we are
currently experiencing is based on our ability to amplify DNA
sequences enzymatically via the Polymerase Chain Reaction (PCR).
Several techniques re1ated to PCR have been developed and used
extensively in ecological and evolutionary studies.
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