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In Vitro Fertilization and Embryo Transfer - A Manual of Basic Techniques (Paperback, Softcover reprint of the original 1st ed. 1988)
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In Vitro Fertilization and Embryo Transfer - A Manual of Basic Techniques (Paperback, Softcover reprint of the original 1st ed. 1988)
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The use of human in vitro fertilization in the management of
infertility is the outgrowth of years of laboratory observations on
in vitro sperm-egg interaction. "The editors of this work have
themselves contributed significantly to basic knowledge of the
mammalian fertilization process. The observations of Don Wolf on
sperm penetration, the block to polyspermy and, most recently,
sperm hyperactivation in the monkey and human, Gregory Kopf's
elucidation of the mechanisms of sperm activation during
penetration and the reciprocal dialogue between sperm and egg, and
Barry Bavister's definition of culture conditions and requirements
necessary for in vitro oocyte maturation, fertilization and
development in model mammalian systems including nonhuman primates
have contributed greatly to our understanding of the mammalian
fertilization process. Wolf, Kopf and Gerrity have enjoyed
substantial interaction with clinicians in Departments of
Obstetrics and Gynecology and have been directly involved with
successful IVF programs. Both Wolf and Kopf have served as research
scientists in the Division of Reproductive Biology at the
University of Pennsylvania, which, for more than 22 years, has
fostered co-mingling of clinically oriented and basic science
faculty. It is through such interaction, which clearly exists at
many institutions including the University of Wisconsin, that the
process of technology transfer is best served. Without an
exquisitely coordinated laboratory, there can be no consistent
success in human in vitro fertilization. Quality control is
pivotal, but close collaboration between the laboratory and the
clinic is also essential as information is shared and correlated.
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