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Computational Design of Ligand Binding Proteins (Hardcover, 1st ed. 2016)
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Computational Design of Ligand Binding Proteins (Hardcover, 1st ed. 2016)
Series: Methods in Molecular Biology, 1414
Expected to ship within 12 - 17 working days
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This volume provides a collection of protocols and approaches for
the creation of novel ligand binding proteins, compiled and
described by many of today's leaders in the field of protein
engineering. Chapters focus on modeling protein ligand binding
sites, accurate modeling of protein-ligand conformational sampling,
scoring of individual docked solutions, structure-based design
program such as ROSETTA, protein engineering, and additional
methodological approaches. Examples of applications include the
design of metal-binding proteins and light-induced ligand binding
proteins, the creation of binding proteins that also display
catalytic activity, and the binding of larger peptide, protein, DNA
and RNA ligands. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and tips on troubleshooting and avoiding known pitfalls.
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