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Books > Science & Mathematics > Biology, life sciences > Biochemistry > Proteins
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Two Hybrid Technologies - Methods and Protocols (Hardcover, 2012)
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Two Hybrid Technologies - Methods and Protocols (Hardcover, 2012)
Series: Methods in Molecular Biology, 812
Expected to ship within 12 - 17 working days
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Protein-protein interactions (PPIs) are strongly predictive of
functional relationships among proteins in virtually all processes
that take place in the living cell. Therefore, the comprehensive
exploration of interactome networks is one of the major goals in
systems biology. The aim of Two Hybrid Technologies: Methods and
Protocols is to provide a compendium of state-of-the art protocols
for the investigation of binary PPIs with the classical yeast
two-hybrid (Y2H) approach, Y2H variants and other in vivo methods
for PPI mapping. Divided into two convenient sections, the first
gives a survey of protocols that are currently employed for Y2H
high-throughput screens by different expert labs in the field.
Rather than detailing the principles of screening, which have been
described previously, the focus is on different implementations of
Y2H interactome mapping. The second section of the book considers
innovative PPI detection methods that have the potential to emerge
as alternative high-throughput methodologies. Written in the highly
successful Methods in Molecular Biology (TM) series format,
chapters contain introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and notes on troubleshooting and
avoiding known pitfalls. Authoritative and accessible, Two Hybrid
Technologies: Methods and Protocols supplies researchers with a
comprehensive toolbox for the identification of biologically
relevant protein interactions.
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