Microscopy, Optical Spectroscopy, and Macroscopic Techniques (Paperback, 1994 ed.)

This is the second of three volumes of Methods in Molecular Biology that deal with Physical Methods of Analysis. The first of these, Spectroscopic Methods and Analyses dealt with NMR spec troscopy, mass spectrometry, and metalloprotein techniques, and the third will cover X-ray crystallographic methods. As with the first volume. Microscopy, Optical Spectroscopy, and Macroscopic Techniques is intended to provide a basic understand ing for the biochemist or biologist who needs to collaborate with spe cialists in applying the techniques of modern physical chemistry to biological macromolecules. The methods treated in this book fall into four groups. Part One covers microscopy, which aims to visualize individual molecules or complexes of several molecules. Electron microscopy is the more familiar of these, while scanning tunneling microscopy is a new and rapidly developing tool. Methods for determining the shapes and sizes of molecules in solution are described in Part Two, which includes chapters on X-ray and neutron scattering, light scattering, and ult- centrifugation. Calorimetry, described in Part Three, provides the means to monitor processes involving thermodynamic changes, whether these are intramolecular, such as conformational transition, or the interactions between solutes or between a solute and its sol vent. Part Four is concerned with optical and infrared spectroscopy and describes applications ranging from the measurement of protein concentration by UV absorbance to the analysis of secondary struc ture using circular dichroism and Fourier-transform infrared spec troscopy.

General

Imprint:	HumanaPress
Country of origin:	United States
Series:	Methods in Molecular Biology, 22
Release date:	September 2014
First published:	1994
Authors:	Christopher Jones • Barbara Mulloy • Adrian H. Thomas
Dimensions:	229 x 152 x 21mm (L x W x T)
Format:	Paperback
Pages:	251
Edition:	1994 ed.
ISBN-13:	978-1-4899-4001-8
Categories:	Books > Science & Mathematics > Chemistry > Analytical chemistry > General
LSN:	1-4899-4001-4
Barcode:	9781489940018

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