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Mitosis - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed. 2014)
Loot Price: R4,265
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Mitosis - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed. 2014)
Series: Methods in Molecular Biology, 1136
Expected to ship within 10 - 15 working days
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Mitosis: Methods and Protocols provides state-of-the-art overviews
on the most important approaches currently used in mitosis research
spanning from the analysis of single molecules in isolation to
their utilization within the complex environment of the cell. The
volume is divided into four parts, each focused on methods
pertaining to distinct aspects of mitosis research. Part I presents
approaches for visualizing and analyzing the dynamic behaviors of
the spindle apparatus, the microtubule based machine that drives
chromosome segregation. Part II focuses more generally on methods
for studying and manipulating the microtubule cytoskeleton in cells
and complex cell free extracts. Part III provides state of the art
biophysical and high resolution microscopy approaches for assessing
complex interactions between microtubules and
microtubule-associated proteins in isolation as well as microtubule
structure in cells. Part IV provides methods for studying the
effects of cell shape on cell division and methods for quantifying
aneuploidy (aberrant chromosome number) which frequently results
from mitotic defects and has been linked to human maladies ranging
from birth defects to cancer. Written in the highly successful
Methods in Molecular Biology series format, chapters include
introductions to their respective topics, lists of the necessary
materials and reagents, step-by-step, readily reproducible
laboratory protocols and key tips on troubleshooting and avoiding
known pitfalls. Authoritative and practical, Mitosis: Methods and
Protocols seeks to provide diverse methods and new techniques to
address new or old questions related to the mechanisms of mitosis.
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