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Books > Science & Mathematics > Biology, life sciences > Biochemistry
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Polyamine Protocols (Paperback, Softcover reprint of hardcover 1st ed. 1998)
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Polyamine Protocols (Paperback, Softcover reprint of hardcover 1st ed. 1998)
Series: Methods in Molecular Biology, 79
Expected to ship within 10 - 15 working days
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Putrescine and spermidine are ubiquitous in living organisms.
Spermine, third of the three most commonly occurring natural
polyamines, is probably present in all eukaryotes but is rare (or
nonexistent) in prokaryotes. Polyamine residues are constituents of
many compounds found in plants and insects. Putrescine, spermidine,
or spermine-containing alkaloids are found in many plants,
nonproteinaceous spider and wasp toxins contain polyamine residues,
and glutathionyl-spermidine conjugates have been found in some
pathogenic microorganisms. In most cells polyamines are the
products of a highly regulated bios- thetic pathway. It is not
clear whether the elaborate regulation of polyamine synthesis is a
consequence of their essential role(s) in cellular differentiation
and development, or part of a defense mechanism to prevent
overaccumulation of compounds that are toxic in excess. In addition
to their biosynthetic capa- bility, many cells also possess
transport systems for polyamines that respond to intracellular
polyamine levels, and other stimuli, and are regulated by mecha-
nisms that are at present incompletely defined. Two routes of
polyamine catabolism have been identified in mammalian cells, a
biodegradative route and a recycling pathway. The relative impor-
tance of these pathways and their overall regulation is only
partially resolved. What is clear is the widespread occurrence of a
variety of polyamine-oxid- ing enzymes in animals, plants,
bacteria, and fungi. Polyamine catabolism, by whichever route,
results in the formation of aminoaldehydes as intermediates.
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