This volume explores the latest methods used to study AMPK by
computational, biochemical, biophysical, cellular, and ex vivo and
in vivo approaches. The chapters in this book cover specific
topics, such as methods to measure change in cellular energy
metabolism and analyze metabolic pathways regulated by AMPK;
bioinformatics tools to identify AMPK targets; knockdown of AMPK by
CRISPR-Cas9; production and crystallization of full-length human
AMP-activated protein kinase; cell-free assays to measure the
effects of regulatory ligands on AMPK; use of sensors of AMPK
activity; AMPK protein interaction by yeast two-hybrid; the role of
AMPK in inflammation and autophagy; analyzing the AMPK function in
C. elegans and mammals (with special focus on skeletal muscle,
blood vessels, kidneys, pancreatic islets and hypothalamus); and
human 2 AMPK mutations. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and tips on troubleshooting and avoiding known pitfalls. Cutting
edge and thorough, AMPK: Methods and Protocols is a valuable
resource for graduate students, postdoctoral researchers, and
established investigators who are interested in the richly diverse
AMPK field.
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