Studies on the effect of recombinant human interferon (rIFN) on the
growth of colonies of multi potential hematopoietic progenitors
have demonstrated that IFN inhibits the growth of these cells in
culture. The inhibitory effect of IFN was more pronounced in
multilineage colony forming cells (CFU-GEMMT) of marrow cells of
patients with chronic myelogenous leukemia (CML) compared with
stern cells of normal marrow. T cell colony formation (CFU-TL) of
phi_positive T cells obtained from primary multilineage
hematopoietic colonies was inhibited by rIFN. Inhibition of T cell
colony formation of Phi T cells was more profound by IFN compared
with secondary T cells of healthy individuals. Southern blot
analysis of secondary T cells from normal individuals revealed a
germ line pattern when examined for T cell receptor (TcR) gene re
arrangements using the cDNA of the beta chain. Subcloned Phi T
cells of one patient with CML demonstrated gene rearrangements of
the TcR. We also demonstrated the disappearance of phi_positive
multilineage hematopoietic colonies when marrow cells were
preincubated with IFN and subsequently cultured in the presence of
IFN. The cytoconversion of the Phi chromosome in metaphases
obtained from individually analyzed multilineage hematopoietic
colonies suggests that IFN might allow the restoration of nonclonal
hematopoiesis in patients with CML. Acknowledgement. Supported in
part by Deutsche Forschungsgemeinschaft and the Medical Research
Council of Canada. References 1. Koeffler HP, Golde DW (1981)
Chronic myelogenous 1eukelnia - new concepts.
General
Is the information for this product incomplete, wrong or inappropriate?
Let us know about it.
Does this product have an incorrect or missing image?
Send us a new image.
Is this product missing categories?
Add more categories.
Review This Product
No reviews yet - be the first to create one!
Welcome to Loot.co.za!
Sign in / Register |Wishlists & Gift Vouchers |Help | Advanced search
