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Hepatoprotective activity of Kamilari (Paperback)
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Hepatoprotective activity of Kamilari (Paperback)
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Master's Thesis from the year 2012 in the subject Pharmicology,
grade: A, Mahatma Ghandi University (University College of
Pharmacy), course: M.Pharm, language: English, abstract: Polyherbal
formulations available with a wide range of indications like
hepatoprotective, appetite and growth promoters, gastrointestinal
and hepatic regulator, as treatment for hepatic dysfunction, for
hepatic regeneration as well as liver stimulant and tonic. Despite
the widespread use, there is a lack of scientific evidence on their
efficacy and safety. This study was designed to evaluate the effect
of Polyherbal formulation Kamialri on oxidative stress and
associated damages in carbon tetrachloride induced toxicity in
Chang liver cells. The study is mainly focused on the evaluation of
its antioxidant potential, invitro steatosis and intracellular
localisation of reduced glutathione by Monochlorobimane(MCB)
staining. Carbon tetrachloride induced toxicity in chang liver
cells was well manifested by significant decrease in cell
viability, enhanced lipid peroxidation leading to tissue damage,
decreased SOD, glutathione, glutathione peroxidase and glutathione
S- transferase and increased deposition of fatty acid . MTT study
revealed that Kamilari has got cytoprotective activity. It has got
the excellent free radical scavenging properties which was proven
by various antioxidant activities (LP, SOD, GSH, GPX, and GST).
Inhibitory effect of Kamilari on fatty acid deposition was proven
by invitro steatosis studies which were conducted on palmitic acid
treated chang cell line.Based on the above studies it is concluded
that Kamilari has cytoprotective and antioxidant activity, and
found to be effective in steatosis.
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