Kary Mullis was awarded a Nobel Prize for inventing the PCR
technique more than 15 years ago in 1993. Since its "discovery,"
multiple adaptations and variations of the standard PCR technique
have been described, with many of these adaptations and variations
currently being used in clinical, diagnostic and academic
laboratories across the world. Further, these techniques are being
applied at the diagnostic level (e.g. as high throughput testing
methodologies to detect minimum residual disease, the
presence/absence of specific pathogens etc), as well as to increase
our understanding of fundamental disease processes.
Frequently, PCR technicians and specialists limit their
understanding of PCR to one particular methodology. However, this
approach limits their appreciation of the range of versatile PCR
techniques currently available, techniques that may be applicable
and indeed more suitable to their own laboratory situation.
This manual aims to provide the reader with a guide to the
standard PCR technique and its many available modifications, with
particular emphasis on the role of PCR techniques in the diagnostic
laboratory (the central theme of this manual). Further, many
important technical issues have been addressed, including types of
PCR template material, PCR optimization, the analysis of PCR
products, quality control and quality assurance, variants and
adaptations of the standard PCR protocol, quantitative PCR and in
situ PCR. The reader of this manual will be excellently informed
about the fundamental principles of PCR and the true potential of
PCR within clinical laboratory practice.
General
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