The ESCRT Complexes - Methods and Protocols (Paperback, 1st ed. 2019)

This detailed collection gathers both established and recent technical procedures to study the Endosomal Sorting Complex Required for Transport (ESCRT) complexes in a wide range of biological systems: Archaea, A. thaliana, U. maydis, S. cerevisiae, S. pombe, C. elegans, D. melanogaster, and mammalian cells. Opening with a section on imaging techniques, the book continues with chapters covering biochemical approaches presenting strategies for production and characterization of recombinant ESCRT proteins, or of specific ESCRT protein domains, as well as genetic and proteomic experimental approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, The ESCRT Complexes: Methods and Protocols serves as a compact guide for researchers interested in establishing an integrated approach to investigate the ESCRT machinery functions in cell biology.

General

Imprint:	HumanaPress
Country of origin:	United States
Series:	Methods in Molecular Biology, 1998
Release date:	August 2020
First published:	2019
Editors:	Emmanuel Culetto • Renaud Legouis
Dimensions:	254 x 178mm (L x W)
Format:	Paperback
Pages:	322
Edition:	1st ed. 2019
ISBN-13:	978-1-4939-9494-6
Categories:	Books > Science & Mathematics > Biology, life sciences > Biochemistry > Proteins Books > Science & Mathematics > Biology, life sciences > Cellular biology > General
LSN:	1-4939-9494-8
Barcode:	9781493994946

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