As the highly anticipated update to Lipid Second Messengers (CRC
Press, 1999), Lipid-Mediating Signaling is a current and
comprehensive overview of research methods used in lipid-mediated
signal transduction. Pioneering experts provide a much-needed
distillation of a decade s worth of advances in research techniques
that are pertinent in understanding how lipid-mediated signal
transduction ties to pathologic disorders.
Part I describes methods used to identify activities of and
assay the expression of different enzymes involved in
lipid-mediated signaling. Part II highlights the use of mass
spectrometry to ascertain the lipid content in various systems.
Part III contains two chapters devoted to techniques used to
determine the role of lipids in the activation of gene
transcription. Part IV rounds out the book s solid coverage by
focusing on methods used to assess fatty acid uptake and
metabolism.
Lipid-Mediating Signaling is an unrivaled reference for this
rapidly expanding, yet resource-limited field, and it is a valuable
resource for for cell biologists, biochemists, and pharmacologists,
as well as for researchers studying signaling transduction.
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