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PRINS & PNA - Technologies in Chromosomal Investigations (Hardcover, Illustrated Ed)
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PRINS & PNA - Technologies in Chromosomal Investigations (Hardcover, Illustrated Ed)
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Total price: R3,323
Discovery Miles: 33 230
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Book & CD. Advances in molecular biotechnology have greatly
improved the sensitivity and the efficiency of methods utilised for
genetic investigations and diagnosis. In the domain of chromosome
analysis, the introduction of molecular techniques has led to the
development of a new approach, called Molecular Cytogenetics, which
has surpassed previously available techniques to become a foremost
biological method. The fluorescence in situ hybridisation (FISH) is
quickly became the standard technique for in situ chromosomal
investigations, as illustrated by its large variety of applications
in research and diagnosis. However, during the last decade,
alternative methods to FISH have been introduced and have shown to
be valuable in detecting chromosomes and quantifying chromosomal
abnormalities. These alternative procedures are the Primed IN Situ
(PRINS) labelling and the Peptide Nucleic Acid (PNA) probes. The
two procedures present several advantages for the in situ detection
of nucleic acid sequences, such as the small size of PNA probes and
PRINS primers, or the fast kinetics of PRINS and PNA labelling
reactions, that make them very attractive for a number of
cytogenetic purposes. This book provides a valuable introduction
and overview of the principles and the applications of alternative
approaches in the field of molecular cytogenetics.
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