"Plant Chemical Genomics: Methods and Protocols ," expert
researchers in the field detail many of the techniques used for
identification of bioactive compounds from a large selection of
fields in plant biology including plant pathogenesis, immune
responses, small RNA processing, endomembrane trafficking, lipids,
plant hormone signaling and cell wall. The presentation of these
and other examples as well as synthetic chemistry, computation and
target identification provides a comprehensive overview of the
practical aspects of chemical biology that are possible in plant
systems. Small molecules and natural products that produce a
phenotype in plants have been known for decades. The agrichemical
industry has utilized small molecules based on known natural
molecules such as auxins, cytokinins, abscissic acid and other
growth regulators. In recent years, the screening and
characterization of novel small molecules has enhanced our
understanding of protein function, metabolic, signaling and
endomembrane pathways, and their interactions in basic plant
research.
Written in the highly successful "Methods in Molecular Biology
"series format, chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and key
tips on troubleshooting and avoiding known pitfalls.
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