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Calcium-Binding Protein Protocols - Volume 1: Reviews and Case Studies (Hardcover)
Loot Price: R3,023
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Calcium-Binding Protein Protocols - Volume 1: Reviews and Case Studies (Hardcover)
Series: Methods in Molecular Biology, 172
Expected to ship within 10 - 15 working days
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Blurb for Volume 1
Hans Vogel and a panel of leading researchers review the protein
chemistry and behavior of this significant protein class, and
provide a comprehensive collection of proven experimental
techniques for their study both in vitro and in vivo. This first
volume discusses the role of calcium in intracellular secondary
messenger activation mechanisms, including unique aspects of
calcium chemistry and its utilization in diary proteins and blood
clotting. Detailed case studies provide a wealth of valuable
information about protein purification and characterization
strategies, X-ray crystallography, and specific calcium-binding
proteins and their modes of action. The second companion volume
focuses on cutting-edge experimental methods for studying solution
structure, stability, dynamics, calcium-binding properties, and
biological activity of calcium-binding proteins in general.
Single Blurb for Both Volumes:
Hans Vogel and a panel of leading researchers review the protein
chemistry and behavior of this significant protein class, and
provide a comprehensive collection of proven experimental
techniques for their study both in vitro and in vivo. The first
volume discusses the role of calcium in intracellular secondary
messenger activation mechanisms, including unique aspects of
calcium chemistry and its utilization in dairy proteins and blood
clotting. Detailed case studies provide a wealth of valuable
information about protein purification and characterization
strategies, X-ray crystallography, and specific calcium-binding
proteins and their modes of action. The second volume focuses on
cutting-edge experimental techniques for studying the solution
structure, stability, dynamics, calcium-binding properties, and
biological activity of calcium-binding protein in general. In
addition to enzymatic assays and more routine spectroscopic and
protein chemistry techniques, there are also NMR approaches,
thermodynamic analyses, kinetic measurements such as surface
plasmon resonance, strategies for amino acid sequence alignments,
and fluorescence methods to study the distribution of calcium and
calcium-binding proteins in cells.
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