Funagl secondary growth is a common post-harvest problem when raw
materials are attacked by filamentous fungi during
storage,processing or handling.The antifungal protein AFP is
secreted from Aspergillus giganteus.It is specifically disturbs the
integrity of fungal cell walls and plasma membranes at low
concentrations.The applicability of AFP at different steps during
malting process, storage of some fruits and vegetables to
efficiently prevent secondary growth of filamentous fungi was
studied.AFP appeared to be very efficient against the main fungal
contaminants.The fungal growth was completely blocked and the
mycotoxin was reduced after addition of AFP.Significant improvement
in quality parameters and antioxidant activity of AFP- treated malt
was observed while chemically treated malts were negatively
affected.In vivo experiments showed that AFP could reduce
post-harvest diseases on tomato and mango fruits caused by
Alternaria alternata.A PCR systems for fungal strains detection and
quantification for HACCP applications were established.AFP is
considered as an excellent biological alternative to combat fungal
growth of filamentous fungi on many foodstuffs.
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