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Books > Science & Mathematics > Biology, life sciences > Biochemistry > Proteins
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Split Inteins - Methods and Protocols (Hardcover, 1st ed. 2017)
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Split Inteins - Methods and Protocols (Hardcover, 1st ed. 2017)
Series: Methods in Molecular Biology, 1495
Expected to ship within 12 - 17 working days
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This volume focuses on applications of split inteins, and the
progress that has been made in the past 5 years on discovery and
engineering of fast and more efficient split inteins. The first few
chapters in Split Inteins: Methods and Protocols explore new
techniques on how to use split inteins for affinity purification of
overproduced proteins, and split-intein based technologies to
prepare cyclic peptides and proteins. The next few chapters discuss
semisynthetic protein trans-splicing using one synthetic intein
piece, synthetic intein-extein pieces used to deliver other cargos
for chemical modification both of purified proteins and of proteins
in living cells, as well as isotopic labeling of proteins for NMR
studies, and a discussion on how protein block copolymers can be
generated by protein trans-splicing to form protein hydrogels. The
last few chapters deal with intein applications in transgenic
plants and conditional inteins that can be regulated in artificial
ways by small molecules or light, a cassette-based approach to
quickly test many intein insertion positions, and a computational
approach to predict new intein split sites (the approach also works
for other proteins). Written in the highly successful Methods in
Molecular Biology series format, chapters include introduction to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Split Inteins: Methods and Protocols is
a valuable resource that will provide guidance toward possibilities
of split intein applications, explore proven and detailed protocols
adaptable to various research projects, and inspire new method
developments.
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