Attempts to transfer useful alien genetic material into wheat
initially involved substitution of alien chromosome from the alien
species into wheat genome. Most of these wheat derivatives have had
limited use in practical breeding because of the linked undesirable
genes on the alien segment which resulted in the loss of yield
and/or quality of the recipient wheats. The amount of alien genetic
material in these lines can be reduced by induction of allosyndetic
recombination. In the earlier studies, cytological and / or
biochemical procedures were used to detect and isolate the
wheat-alien recombinant chromosomes. Because of large amount of
time and effort required and the limited resolving power of the
techniques, these procedures though used successfully by the
pioneer workers have not proved very efficient for the
identification and isolation of such recombinants. During present
work, DNA based Co-dominant markers (RFLPs) were used for isolation
and characterization of wheat-Ag. intermedium recombinant
chromosomes. Pattern of homoeologous chromosome recombination was
compared with homologous recombination in Triticeae.
General
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