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Epstein Barr Virus - Methods and Protocols (Hardcover, 1st ed. 2017)
Loot Price: R4,190
Discovery Miles 41 900
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Epstein Barr Virus - Methods and Protocols (Hardcover, 1st ed. 2017)
Series: Methods in Molecular Biology, 1532
Expected to ship within 12 - 17 working days
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This volume explores data from the applications of molecular
biological methods and the applications of recent immunological and
cytogenetic methods in Epstein-Barr Virus (EBV) that will offer
readers possible new solutions to the unresolved problems in the
EBV field. Chapters in this book cover topics such as: viral life
cycle, latency, EBV-associated diseases and EBV diagnostics; in
vitro methods including organotypic cultures for the analysis of
EBV-epithelial cell interactions; identification of the interacting
viral and cellular proteins using affinity purification-mass
spectrometry methods; 3D telomere FISH; transcription analysis
using high-throughput RNA sequencing, qPCR and nuclear run-on
assay; analysis of viral and cellular microRNAs; isolation and
characterization of exosomes and the assessment of their function;
characterization of the viral genome by terminal repeat analysis
and sequencing; the use of chromatin immunoprecipitation coupled
sequencing (ChIP-Seq) for the analysis of Zta-DNA interactions;
epigenetic analysis by bisulfite sequencing and ChIP; novel in vivo
models for the study of EBV infection; and how immunological,
virological, tissue culture and molecular methods can be combined
to yield Good Manufacturing Practice-compliant EBV-specific T cells
for the immunotherapy of EBV-associated post-transplant
lymphoproliferative diseases (PTLD). Written in the highly
successful Methods in Molecular Biology series format, chapters
include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls. Cutting-edge and comprehensive, Epstein
Barr-Virus: Methods and Protocols is a valuable resource for anyone
who is interested in this fascinating and evolving field.
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