The First Book on CRS Microscopy
Compared to conventional Raman microscopy, coherent Raman
scattering (CRS) allows label-free imaging of living cells and
tissues at video rate by enhancing the weak Raman signal through
nonlinear excitation. Edited by pioneers in the field and with
contributions from a distinguished team of experts, Coherent Raman
Scattering Microscopy explains how CRS can be used to obtain a
point-by-point chemical map of live cells and tissues.
In color throughout, the book starts by establishing the
foundation of CRS microscopy. It discusses the principles of
nonlinear optical spectroscopy, particularly coherent Raman
spectroscopy, and presents the theories of contrast mechanisms
pertinent to CRS microscopy. The text then provides important
technical aspects of CRS microscopy, including microscope
construction, detection schemes, and data analyses. It concludes
with a survey of applications that demonstrate how CRS microscopy
has become a valuable tool in biomedicine.
Due to its label-free, noninvasive examinations of living cells
and organisms, CRS microscopy has opened up exciting prospects in
biology and medicine from the mapping of 3D distributions of small
drug molecules to identifying tumors in tissues. An in-depth
exploration of the theories, technology, and applications, this
book shows how CRS microscopy has impacted human health and will
deepen our understanding of life processes in the future."
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