Carotenoids are a family of yellow to orange-red terpenoid
pigments synthesized by photosynthetic organisms and many bacteria
and fungi. They have beneficial health effects protecting against
oxidative damage and may be responsible for the colours associated
with plants and animals. In "Microbial Carotenoids from Bacteria
and Microalgae: Methods and Protocols, "expert researchers in the
field detail many of the most up-to-date methods which are now
commonly used to study carotenoids. These include methods for the
study of canthaxanthin production, construction of carotenoid
reporter systems, directed evolution of carotenoid synthases, and
improvement of b-carotene hydroxylase catalytic activity are
described. Additionally, the book includes methods of DNA
fingerprinting for the identification of carotenogenic "Dunaliella"
species, ketocarotenoid biosynthesis in microalgae expressing the
beta-C-4-carotene oxygenase gene, characterization of
carotenogenesis genes in "Anabaena" sp., obtaining lutein from
microalgal biomass, NMR-based isotopologue profiling of microbial
carotenoids, and analysis of diapocarotenoids. Written in the
highly successful "Methods in Molecular Biology " series format,
chapters include introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and key tips on troubleshooting
and avoiding known pitfalls.
Authoritative and practical, " Microbial Carotenoids from
Bacteria and Microalgae: Methods and Protocols "provides practical
experimental laboratory procedures for a wide range of carotenoids
producing microorganisms."
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