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Books > Science & Mathematics > Biology, life sciences > Life sciences: general issues > Genetics (non-medical) > DNA
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Plant Functional Genomics - Methods and Protocols (Hardcover, 2nd ed. 2015)
Loot Price: R6,001
Discovery Miles 60 010
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Plant Functional Genomics - Methods and Protocols (Hardcover, 2nd ed. 2015)
Series: Methods in Molecular Biology, 1284
Expected to ship within 10 - 15 working days
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This second edition volume discusses the revolutionary development
of faster and less expensive DNA sequencing technologies from the
past 10 years and focuses on general technologies that can be
utilized by a wide array of plant biologists to address specific
questions in their favorite model systems. This book is organized
into five parts. Part I examines the tools and methods required for
identifying epigenetic and conformational changes at the
whole-genome level. Part II presents approaches used to determine
key aspects of a gene's function, such as techniques used to
identify and characterize gene regulatory networks. This is
followed by a discussion of tools used to analyze the levels of
mRNA, mRNA translation rates and metabolites. Part III features a
compilation of forward and reverse genetic approaches that include
recent implementation of high-throughput sequencing in classical
methodologies such as QTL mapping. The final two parts explore
strategies to facilitate and accelerate the generation and testing
of functional DNA elements and basic computational tools used to
facilitate the use of systems biology approached by a broad
spectrum of plant researchers. Written in the highly successful
Methods of Molecular Biology series format, chapters include
introductions to their respective topics, lists of the necessary
materials and reagents, step-by-step, readily reproducible
laboratory protocols and key tips on troubleshooting and avoiding
known pitfalls. Practical and timely, Plant Functional Genomics:
Methods and Protocols, Second Edition highlights the latest
developments in DNA sequencing technologies that are likely to
continue shaping the future of functional genomics.
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