Display technologies have become a very powerful way of
generating therapeutic lead molecules and specific reagents for
increasing our understanding of biology; however, despite being
first described shortly after phage display, the use of ribosome
display and related methods have been much less widespread. Since
this is in part due to the complexity of the methods, "Ribosome
Display and Related Technologies: Methods and Protocols" seeks to
extend their use by collecting expert contributions describing
these detailed protocols. The protocols described range from
well-established methods that have been used for a decade to
generate high affinity antibodies, which are already in the clinic,
to methods that are in their early stages of application such as
display of peptides incorporating non-canonical amino acids.
Written in the highly successful "Methods in Molecular Biology "
series format, chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls.
Invaluable and easy to use, "Ribosome Display and Related
Technologies: Methods and Protocols" will be of great benefit to
those with general molecular biology or protein engineering
experience who wish to select peptides or proteins by display,
those with phage display experience who would benefit from the
application of ribosome display, as well as those with some
ribosome display experience who would like to expand the range of
applications to which they are applying the technology."
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