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Books > Science & Mathematics > Biology, life sciences > Biochemistry > Proteins
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Quantitative Methods in Proteomics (Hardcover, 2012 ed.)
Loot Price: R3,085
Discovery Miles 30 850
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Quantitative Methods in Proteomics (Hardcover, 2012 ed.)
Series: Methods in Molecular Biology, 893
Expected to ship within 10 - 15 working days
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Protein modifications and changes made to them, as well as the
quantities of expressed proteins, can define the various functional
stages of the cell. Accordingly, perturbations can lead to various
diseases and disorders. As a result, it has become paramount to be
able to detect and monitor post-translational modifications and to
measure the abundance of proteins within the cell with extreme
sensitivity. While protein identification is an almost routine
requirement nowadays, reliable techniques for quantifying
unmodified proteins (including those that escape detection under
standard conditions, such as protein isoforms and membrane
proteins) is not routine. Quantitative Methods in Proteomics gives
a detailed survey of topics and methods on the principles
underlying modern protein analysis, from statistical issues when
planning proteomics experiments, to gel-based and mass
spectrometry-based applications. The quantification of
post-translational modifications is also addressed, followed by the
"hot" topics of software and data analysis, as well as various
overview chapters which provide a comprehensive overview of
existing methods in quantitative proteomics. Written in the
successful Methods in Molecular Biology (TM) series format,
chapters include introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily
reproducible protocols, and notes on troubleshooting and avoiding
known pitfalls. Authoritative and easily accessible, Quantitative
Methods in Proteomics serves as a comprehensive and competent
overview of the important and still growing field of quantitative
proteomics.
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