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Recombinant Proteins From Plants - Methods and Protocols (Hardcover, 2009 ed.) Loot Price: R3,003
Discovery Miles 30 030
Recombinant Proteins From Plants - Methods and Protocols (Hardcover, 2009 ed.): Loic Faye, Veronique Gomord

Recombinant Proteins From Plants - Methods and Protocols (Hardcover, 2009 ed.)

Loic Faye, Veronique Gomord

Series: Methods in Molecular Biology, 483

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Loot Price R3,003 Discovery Miles 30 030 | Repayment Terms: R281 pm x 12*

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Altogether, the biochemical, technical and economic limitations on existing proka- otic and eukaryotic expression systems and the growing clinical demand for complex therapeutic proteins have created substantial interest in developing new expression systems for the production of therapeutic proteins. To that end, plants have emerged in the past decade as a suitable alternative to the current production systems, and today their potential for production of high quality, much safer and biologically active complex recombinant pharmaceutical proteins is largely documented. The chapters in this volume, contributed by leaders in the field, sum up the state-- the-art methods for using a variety of different plants as expression hosts for phar- ceutical proteins. Several production platforms are presented, ranging from seed- and leaf-based production in stable transgenic plant lines, to plant cell bioreactors, to viral or Agrobacterium-mediated transient expr ession systems. Currently, antibodies and their derived fragments represent the largest and most important group of biote- nological products in clinical trials. This explains why the potential of most prod- tion platforms is illustrated here principally for antibodies or antibody fragments with acknowledged potential for immunotherapy in humans. In addition, a comparison of different plant expression systems is presented using aprotinin, a commercial phar- ceutical protein, as a test system. Although multiple books and monographs have been recently published on mol- ular pharming, there is a noticeable dearth of bench step-by-step protocols that can be used quickly and easily by beginners entering this new field.

General

Imprint: HumanaPress
Country of origin: United States
Series: Methods in Molecular Biology, 483
Release date: November 2008
First published: 2009
Editors: Loic Faye • Veronique Gomord
Dimensions: 276 x 203 x 23mm (L x W x T)
Format: Hardcover
Pages: 358
Edition: 2009 ed.
ISBN-13: 978-1-58829-978-9
Categories: Books > Professional & Technical > Biochemical engineering > Biotechnology > General
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LSN: 1-58829-978-3
Barcode: 9781588299789

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