Even though they are present in nature, non-proteinogenic amino
acids are usually defined as unnatural or non-natural. Beside their
structural diversity, interest in these compounds is due to their
occurrence in nature, their biological properties, the analytical
aspects, their use as probes, and their incorporation into peptides
and proteins, among other reasons. Divided into five convenient
sections, "Unnatural Amino Acids: Methods and Protocols" deals with
enzymatic methods used to produce non-natural amino acids, aspects
concerning the presence of unnaturalamino acidsin peptides with
antimicrobial properties, genetic incorporation of unnatural amino
acidsinto proteins (yeast and mammalian cells), and detection and
quantification of D-amino acids and related enzymes. Written in the
highly successful "Methods in Molecular Biology " series format,
chapters contain introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and notes on troubleshooting and
avoiding known pitfalls.
Authoritative and accessible, "Unnatural Amino Acids: Methods
and Protocols" serves as an ideal guide for scientists and
contributes to directing the attention of researchers to the many
fields of growing scientific interest in non-natural amino acids.
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