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Books > Science & Mathematics > Biology, life sciences > Microbiology (non-medical)
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Dictyostelium discoideum Protocols (Paperback, Softcover reprint of the original 2nd ed. 2013)
Loot Price: R4,738
Discovery Miles 47 380
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Dictyostelium discoideum Protocols (Paperback, Softcover reprint of the original 2nd ed. 2013)
Series: Methods in Molecular Biology, 983
Expected to ship within 10 - 15 working days
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Dictyostelium discoideum is a well-establish eukaryotic model
organism that offers unique advantages for studying fundamental
cellular processes, including signal transduction, random and
directed cell motility, cytokinesis, endocytosis and vesicle
transport and development. Dictyostelium is also increasingly used
for the investigation of human disease genes and the crosstalk
between host and pathogen. The availability of the genome sequence
at a mouse click together with a whole range of supporting
information and resources along with a powerful armoury of
molecular genetics techniques have considerably enhanced the
experimental attractiveness of D. discoideum in recent years. The
second edition of Dictyostelium discoideum Protocols incorporates
the most recent developments in a number of fields. The book is
divided into four parts. The first part provides an introduction to
the amoebozoa and community resources. The second part presents
large-scale analysis methods made possible by the completion of the
Dictyostelium genome sequence. The third part is dedicated to
molecular genetics techniques, cell biological, biochemical and
biophysical methods. The chapters in the fourth part describe the
use of Dictyostelium as a model system to study vesicle formation,
trafficking and infection by bacterial pathogens. Written in the
highly successful Methods in Molecular Biology (TM) series format,
chapters include introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and key tips on troubleshooting
and avoiding known pitfalls. Authoritative and practical,
Dictyostelium discoideum Protocols, Second Edition seeks to aid
scientists in the application of innovating techniques to study a
range of fundamental biological processes in this attractive model
organism.
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