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Dictyostelium discoideum Protocols (Paperback, Softcover reprint of hardcover 1st ed. 2006)
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Dictyostelium discoideum Protocols (Paperback, Softcover reprint of hardcover 1st ed. 2006)
Series: Methods in Molecular Biology, 346
Expected to ship within 10 - 15 working days
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Dictyostelium discoideum is a simple but fascinating eukaryotic
microorg- ism, whose natural habitat is deciduous forest soil and
decaying leaves, where the amoebae feed on bacteria and grow as
independent single cells. Exhaustion of the bacterial food source
triggers a developmental program, in which up to 100,000 cells
aggregate by chemotaxis towards cAMP. Morphogenesis and cell
different- tion then culminate in the production of spores enabling
the organism to survive unfavorable conditions. Dictyostelium
offers unique advantages for studying f- damental cellular
processes with the aid of powerful molecular genetic, bioche- cal,
and cell biological tools. These processes include signal
transduction, chemotaxis, cell motility, cytokinesis, phagocytosis,
and aspects of development such as cell sorting, pattern formation
and cell type differentiation. Recently, D- tyostelium was also
described as a suitable host for pathogenic bacteria in which one
can conveniently study the process of infection. In addition,
Dictyostelium has many of the experimental conveniences of
Saccharomyces cerevisiae and is pr- ably the best experimentally
manipulatable protozoan, providing insight into this diverse group
of organisms, which includes some of the most dangerous human
parasites. The recent completion of the Dictyostelium genome
sequencing project strengthens the position of D. discoideum as a
model organism. The completed genome sequence and other valuable
community resources constitute the source for basic biological and
biomedical research and for genome-wide analyses.
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