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Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas - Molecular Genetics; Lung and Breast Carcinomas (Hardcover, New)
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Discovery Miles 53 110
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Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas - Molecular Genetics; Lung and Breast Carcinomas (Hardcover, New)
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The various cell types have traditionally been recognized and
classified according to their appearance in the light microscope
following the process of fixing, processing, sectioning, and
staining tissues that is known as histology. Classical histology
has been augmented by
immunohistochemistry (the use of specific antibodies to stain
particular molecular species "in situ"). Immunohistochemistry has
allowed the identification of many more cell types than could be
visualized by classical histology, particularly in the immune
system and among the scattered hormone-secreting cells of the
endocrine system.
This book discusses all aspects of immunohistochemistry and "in
situ" hybridization technologies and the important role they play
in reaching a cancer diagnosis. It provides step-by-step
instructions on the methods of additional molecular technologies
such as DNA microarrays, and microdissection, along with the
benefits and limitations of each method. The topics of
region-specific gene expression, its role in cancer development and
the techniques that assist in the understanding of the molecular
basis of disease are relevant and necessary in science today,
ensuring a wide audience for this book.
* The only book available that translates molecular genetics into
cancer diagnosis
* Provides the readers with tools necessary to perform and optimize
sensitive, powerful techniques, including immunohistochemistry and
fluorescence in situ hybridization, used in tumor diagnosis
* Written by experts in this field, the book provides theoretical
considerations as well as practical approaches to carry out
effectively these techniques
* Offers suggestions, tips, cautions, and guidelines to avoid
artifacts and misdiagnosis
* Introduces new techniques to detect genes and proteins involved
in the initiation and progression of cancer
* Covers the latest developments and a wide range of applications
to the detection of antigens and single-copy DNA and RNA
* Written in a uniform format, each chapter includes Introduction,
Materials required, step-by-step detailed Methods, Results,
Discussion, and comprehensive up-to-date References
General
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