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Books > Science & Mathematics > Biology, life sciences > Life sciences: general issues > Genetics (non-medical) > DNA
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Tag-based Next Generation Sequencing (Hardcover)
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Tag-based Next Generation Sequencing (Hardcover)
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Tag-based approaches were originally designed to increase the
throughput of capillary sequencing, where concatemers of short
sequences were first used in expression profiling. New Next
Generation Sequencing methods largely extended the use of tag-based
approaches as the tag lengths perfectly match with the short read
length of highly parallel sequencing reactions. Tag-based
approaches will maintain their important role in life and
biomedical science, because longer read lengths are often not
required to obtain meaningful data for many applications. Whereas
genome re-sequencing and de novo sequencing will benefit from ever
more powerful sequencing methods, analytical applications can be
performed by tag-based approaches, where the focus shifts from
'sequencing power' to better means of data analysis and
visualization for common users. Today Next Generation Sequence data
require powerful bioinformatics expertise that has to be converted
into easy-to-use data analysis tools. The book's intention is to
give an overview on recently developed tag-based approaches along
with means of their data analysis together with introductions to
Next-Generation Sequencing Methods, protocols and user guides to be
an entry for scientists to tag-based approaches for Next Generation
Sequencing.
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