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Books > Science & Mathematics > Biology, life sciences > Cellular biology
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The Ubiquitin Code (Hardcover, 1st ed. 2023)
Loot Price: R4,995
Discovery Miles 49 950
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The Ubiquitin Code (Hardcover, 1st ed. 2023)
Series: Methods in Molecular Biology, 2602
Expected to ship within 10 - 15 working days
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Total price: R5,015
Discovery Miles: 50 150
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This volume details novel and popular approaches to decipher the
Ubiquitin Code. Chapters guide readers through ubiquitin chain
complexity, methods to generate non-hydrolysable ubiquitin-NEDD8, a
fully synthetic method for the linear synthesis of fluorescently
labelled rhodamine LC3A and LC3B, a HECT ligase-mediated
ubiquitylation in vitro, a RNAi-based screening to identify DUBs, a
Yuh1 activity assay, a pull-down to isolate K48 and K63 chains
using specific nanobodies, identification of NeDDylated protein
based in a NEDD8R74K mutant, in vivo identification of NEDDylated
proteins from liver diseases, UbL-ID method to identify
ubiquitin-like modified proteins, a LC3/GABARAP capturing system,
and methods to isolate peptides that are closely associated with
proteasomes or trapped inside the core particle of proteasomes in
eukaryotic cells, among others. Written in the successful Methods
in Molecular Biology series format, chapters include introductions
to their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible protocols, and notes
on troubleshooting and avoiding known pitfalls. Authoritative and
cutting-edge, The Ubiquitin Code aims to to be a useful practical
guide to researches to help further their study in this field.
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