Pear belongs to genus Pyrus, abundantly found in temperate region
of AJ & K reflects rich biodiversity. Such diversity and
valuable genetic resources are being eroded due to human over
exploitation, and natural calamities. Keeping in view existing
genetic diversity and genetic erosion in Pyrus germplasm, the focus
of present study was to develop biotechnological conservation
strategies by using slow-growth techniques. However in vivo
preservation was also implemented to conserve the basic stock under
field conditions. The apical shoots of nine pear genotypes were
preserved in vitro under minimal growth conditions using low
temperature treatments, modification in medium strength and adding
mannitol in the basal medium and also by reducing the concentration
of BAP and using growth retardants for different storage periods
i.e. 3, 6, 9 and 12 months. After twelve months of storage, the
culture successfully regenerated into new shoots and they were
morphologically similar to those of non-stored.
General
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