This volume, with chapters written by experts in the field of
cancerous tumors, details the key factors associated with liquid
biopsies in solid tumors: blood-based diagnostics; circulating
tumor cells; enumeration and molecular analysis (association with
breast cancer); epithelialmesenchymal transition; detection and
monitoring; circulating-free tumor DNA; CTCs and ctDNA; and the
exosome. The field of blood-based diagnostics is rapidly evolving
demonstrating the possibility of real-time molecular analysis of
cancer cells and their phenotype and genotype. Circulating Tumor
Cell (CTCs) have demonstrated prognostic and predictive value in
advanced cancer and represents a source of tumor cells for
transcriptome and genomic analysis. Most recently, the detection of
genomic abnormalities in the peripheral blood by sensitive and
selective PCR methods (liquid biopsy) opened to the option of a
comprehensive blood-based tumor analysis. Similar information can
be obtained by analysis of exosome, a natural packaging and
messaging system being explored in advanced malignancies. The final
frontier is the evaluation of immune cells determinant of innate
and adaptive immunity.
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