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Nucleic Acid Biosensors for Environmental Pollution Monitoring (Hardcover)
Loot Price: R4,265
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Nucleic Acid Biosensors for Environmental Pollution Monitoring (Hardcover)
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Nucleic acids are the fundamental building blocks of life and are
found in all living things. In recent years, their functions have
been shown to extend beyond the Watson-Crick base pair recognition
of complementary strands. Molecules (known as aptamers) consisting
of 40-50 nucleotides have been isolated that are able to bind a
broad range of molecules with high affinity and specificity. The
molecules recognized by aptamers range from small organic molecules
to proteins, cells and even intact viral particles. Catalytic DNA
molecules called NAzymes (RNAzyme or DNAzyme) have also been shown
to exist and, when combined with aptamers, are known as aptazymes.
These biomolecules can be used to develop smart and innovative
biosensors for environmental analysis. Monitoring of contaminants
in the air, water and soil is a key component in understanding and
managing risks to human health and ecosystems. This, in conjunction
with the time and cost involved in traditional chemical analysis,
means there is a growing need for simple, rapid, cost-effective and
portable screening methods. Biosensors are compact devices which
complement current field screening and monitoring methods. This
book demonstrates the incredible opportunities that nucleic acids
can offer to environmental analytical chemistry. The chapters: show
how nucleic acids have a pivotal role in the development of smart
biosensors for environmental monitoring; describe the development
of biosensors based on aptamers and NAzymes for the detection of
organic and inorganic pollutants; deal with the use of nucleic acid
based biosensors for environmental toxicity screening, and detail
the use of nanomaterials, as well as miniaturization and
lab-on-a-chip technologies, for nucleic acid based biosensing
systems.
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