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Books > Science & Mathematics > Biology, life sciences > Life sciences: general issues > Genetics (non-medical)
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Expression Systems - Methods Express (Paperback)
Loot Price: R1,215
Discovery Miles 12 150
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Expression Systems - Methods Express (Paperback)
Series: Methods Express Series
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Protein expression is an increasingly important tool for research
on gene function. What is needed is not just a lab manual providing
established methods as well as the latest state-of-the-art
protocols, but also clear advice on what expression system to
choose when. Expression Systems: Methods Express uniquely fills
this need. It covers expression across a broad range of systems,
including the following: Baculovirus expression vectors CHO cells
E. coli HEK293-EBNA1 cells Lactococcus lactis and other gram
positive bacteria S. cerevisiae Transfected insect cells Pichia
pastoris Mammalian cells using BacMam viruses Lentiviral vectors
Wheat germ cell-free system The book takes the reader through how
to make an informed choice of appropriate system, taking into
account the protein target, the time involved, the ultimate use of
the expressed protein, and the laboratory equipment required. It
also provides step-by-step methods for each system. In addition,
the book describes the optimisation of expression strategies,
expression engineering using ribosome display, and how to select
protein variants with improved expression. Every chapter discusses
the merits and limitations of the approaches available, describes
the key techniques in full practical detail, and provides sensible
advice for immediate use at the bench. In summary, Expression
Systems: Methods Express is a comprehensive laboratory manual and
information resource for researchers at all levels, from
postgraduate student to principal investigator.
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