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Bone Research Protocols (Hardcover, 2nd ed. 2012)
Loot Price: R4,742
Discovery Miles 47 420
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Bone Research Protocols (Hardcover, 2nd ed. 2012)
Series: Methods in Molecular Biology, 816
Expected to ship within 12 - 17 working days
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Studies over the past decade have continued to bring tremendous
advances to our understanding of bone biology. New pathways have
been discovered and expanded our knowledge of the ways in which
genes and gene products affect bone cells and thereby bone mass and
bone strength. In Bone Research Protocols, Second Edition, expert
researchers in the field detail many methods commonly used to study
bone biology. Focusing mainly on in vitro methods, this volume
gives techniques for isolation, culture and functional analysis of
all bone cell types and details a range of imaging methods,
including light and ultrastructural microscopy and live cell
imaging. Some important in vivo techniques are included, such as
analysis of bone resorption and imaging using X rays, fluorescent
or luminescent techniques. Methods for study of proteins and
nucleic acid are included and methods for analysis of bone
composition, measurement of bone strength, and response to
mechanical stimulation are described. Written in the highly
successful Methods in Molecular Biology (TM) series format,
chapters include introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and key tips on troubleshooting
and avoiding known pitfalls. Authoritative and practical, Bone
Research Protocols, Second Edition seeks to aid scientists in the
bone field to establish new techniques in their laboratories.
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