Pakistan has vast population of dogs belonging to different
breeds.Most of the dogs have no pedigree record which is a great
threat to conservation of different breeds.No study on DNA
fingerprinting of dogs has been conducted in Pakistan.DNA
fingerprinting of dogs is necessary to overcome the problems like
forensic cases, individual identity in case of fertilization by
more than one male and ownership disputes.Microsatellite markers
have been proved as an efficient tool for parentage testing and
breed characterization of dogs. In this study, a panel of
microsatellite markers was developed.Blood samples were taken from
cephalic vein of two breeds of dogs(German shepherd and Labrador
retriever). DNA was extracted and primers were optimized.Multiplex
PCR was performed and genotyping was done for the PCR products on
non denaturing polyacrylamide gel.These results were analyzed
statistically.Almost all of the microsatellite markers showed
significant variations in both breeds.Results of this study lead to
development of a panel of microsatellite markers which can be used
for parentage analysis and breed characterization of dog
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