Tuberculosis is devastating disease at global level. TB is caused
by members of Mycobaterium tuberculosis complex group. Presently
available biochemical methods of diagnosis of tuberculosis are not
accurate. The highly sensitive polymerase chain reaction (PCR) and
specific molecular probes techniques are major advances in early
diagnosis and molecular epidemiology of various diseases including
TB. It is established that the ribosomal gene region of both the
prokaryotic and eukaryotic comprise of sequences that are conserved
during evolution interspersed with sequence which are divergent.
The analysis of the 16S rRNA gene promoter region for rapid and
accurate differentiation and identification of Mycobacterial
species and understanding of their phylogenetic relationships is
likely to prove to be advantageous over the use of previously used
target genes. The resulting sensitivity is likely to allow the
generation of RFLP patterns in a matter of hours to differentiate
not only between the species investigated in the present study but
others as well, by possible detection of novel RFLP patterns. For
unidentified cases, PCR product could be characterized by
subsequent sequencing.
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