Bone marrow stem cells are the most transplanted cells
worldwide. These cells are used as a replacement therapy for
patients suffering from a diverse number of hematopoietic diseases
and immunodeficiencies. However, the use of bone marrow cells in
regenerative medicine has so far remained without much success. In
the new era of pluripotent stem cells, great opportunities for
establishing new therapies have opened up. The discovery of human
embryonic stem cells and that of induced pluripotent (iPS) stem
cells has made it possible to derive any desired tissues for
regenerative medicine as iPS cell derived cells are only limited by
the lack of established protocols that can be applied in humans.
There is no doubt that stem cells present a new and innovative
platform for establishing novel cell based therapies. The challenge
is to establish new protocols that allow the successful
differentiation of these cells into lineage committed cells.
Embryonic Stem Cell Immunobiology: Methods and Protocols covers a
variety of relevant topics, such as hematopoietic stem cells
derived from ES cells, the interaction of these cells with natural
killer cells or with cytotoxic T cells, and specific protocols for
the derivation of hematopoietic cells and neuronal cells, to name a
few. Written in the highly successful Methods in Molecular Biology
series format, chapters contain introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and notes
on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Embryonic Stem Cell Immunobiology:
Methods and Protocols serves as an ideal guide to experts and
non-experts interested in different aspects of stem cells.
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