Every cell in our bodies contains a great variety and number of
permeability pathways for various organic and inorganic ions,
water, metabolites, nutrients, and signalling molecules.
Maintenance and precise control of gating within these pathways are
fundamental principles of life as these underlie basic cellular
functions such as communication, contractility, and metabolism. In
Ion Channels: Methods and Protocols, Second Edition, experts in the
field contribute chapters that focus on the strategies, approaches,
methods, and protocols for studying a large family of proteins that
form ionic channels in the plasma membrane and intracellular
membranes of cells. Using practical examples from the cutting-edge
current research, this volume will take a look back at the major
methods and approaches that aided the progress toward the current
understanding of ion channel function, structural design, and
biological roles. The volume also aims to look forward and identify
approaches that will lead us to future discoveries. Written in the
highly successful Methods in Molecular Biology (TM) series format,
chapters include introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls. Authoritative and cutting-edge, Ion
Channels: Methods and Protocols, Second Edition will greatly assist
researchers searching for specific methodology in studying ion
channels on the path toward a greater understanding of these key
biological features.
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