Reactive dyes containing diazo bonds are more resistant to
anaerobic reduction in comparison to monoazo dyes. Reactive
Yellow-145 (RY-145) which is used as a model dye is a diazo dye
having -SO3H group in its structure. A novel bacterial consortium
was isolated from the soil samples of a local dye manufacturing
unit at Ahmedabad, Gujarat, India. This culture was used for
decolorization parametric study of RY-145 under various operating
conditions. The consortium was successfully able to decolorize, 300
mg l-1 of selected dye up to 97% within 11 h under static
facultative conditions at pH 7.0 and 37 C temperature. The UV -
Visible Spectroscopic analysis results indicate the decolorization
of dye. The HPLC and FTIR results confirm degradation of RY-145
into smaller compounds. For the commercial viability, combination
of specially prepared synthetic medium and extract of agriculture
waste is used as a growth medium for consortium in place of
nutrient broth. COD reduction of nearly 70% is achieved for 20
times diluted industrial wastewater. Specially designed continuous
column reactor (PMSCCR) has also been used for batch and continuous
study of decolorization of RY-145.
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