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Peptide Nucleic Acids - Methods and Protocols (Hardcover, 2002 ed.)
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Peptide Nucleic Acids - Methods and Protocols (Hardcover, 2002 ed.)
Series: Methods in Molecular Biology, 208
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Total price: R3,260
Discovery Miles: 32 600
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Peptide nucleic acids (PNAs) have now existed for slightly more
than ten years, with the interest in and applications of this
pseudopeptide DNA mimic steadily increasing during the entire
period. PNAs have rapidly attracted the attention of scientists
from a diversity of fields ranging from (bio)organic and
biophysical chemistry to prebiotic evolution, and from molecular
biology to genetic diagnostics and drug development. Many of the
applications take advantage of the unique properties of PNA-an
uncharged pseudopeptide-that distinguish this DNA mimic from more
traditional DNA analogs. Rather than trying to create a
comprehensive collection of all published methods and protocols
involving PNA-many of which have not yet been validated- I have
decided to concentrate on select protocols that are either very
well established by several groups around the world, such as
PCR-clamping and in situ hybridization, or on new methods that may
have broader future impact. Basic methods for PNA oligomer
synthesis and analyses have also been included. I am very grateful
to those friends and colleagues who have enthusiastically
contributed their work, discussions, and writing, and thereby made
this book possible. Peter E. Nielsen v Contents Preface. . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . v Contributors. . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . ix IINTRODUCTION 1 PNA
Technology Peter E. Nielsen. . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . 3 II CHEMISTRY 2 Solid Phase Synthesis of
PNA Oligomers Frederik Beck. . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . 29 3 Synthesis of PNA-Peptide Conjugates
Satish Kumar Awasthi and Peter E. Nielsen. . . . . . . . . . . . .
. . . . . 43 4 Parallel Synthesis of PNA-Peptide Conjugate
Libraries Satish Kumar Awasthi and Peter E. Nielsen. . . . . . . .
. . . . . . . . . .
General
Imprint: |
HumanaPress
|
Country of origin: |
United States |
Series: |
Methods in Molecular Biology, 208 |
Release date: |
July 2002 |
First published: |
2002 |
Editors: |
Peter E. Nielsen
|
Dimensions: |
228 x 148 x 20mm (L x W x T) |
Format: |
Hardcover
|
Pages: |
274 |
Edition: |
2002 ed. |
ISBN-13: |
978-0-89603-976-6 |
Categories: |
Books >
Science & Mathematics >
Biology, life sciences >
Molecular biology
|
LSN: |
0-89603-976-5 |
Barcode: |
9780896039766 |
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