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Plant Transformation via Agrobacterium Tumefaciens - Culture Conditions, Recalcitrance and Advances in Soybean (Paperback)
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Plant Transformation via Agrobacterium Tumefaciens - Culture Conditions, Recalcitrance and Advances in Soybean (Paperback)
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Plant Transformation via Agrobacterium Tumefaciens compiles
fundamental and specific information and procedures involving in
vitro soybean transformation, which forms the basis for the
Agrobacterium-mediated genetic manipulation of soybean using plant
tissue culture. This method serves as one of the most preferred,
reliable and cost-effective mechanism of transgene expression in
both leguminous recalcitrant species and non-legume crops. The
technology is favoured due to its simplicity, feasibility and high
transformation rates that are so far achieved mostly in monocot
plants and a few dicot genotypes. This book provides a
comprehensive review of plant transformation which remains
necessary for many researchers who are still facing
protocol-related hurdles. Among some of the major topics covered in
Plant Transformation via Agrobacterium Tumefaciens are the history
and discovery of Agrobacterium bacterium, longstanding challenges
causing transformation inefficiencies, types and conditions of
explants, development of transgenic plants for stress resistance,
and the role of transgenic plants on animal/human health, including
the environment. Plant Transformation via Agrobacterium Tumefaciens
helps the reader to understand how soybean, like many other orphan
legume crops, faces the risk of overexploitation which may render
the currently available varieties redundant and extinct should its
narrow gene pool not improve. Plant transformation serves as a key
technique in improving the gene pool, while developing varieties
that are drought tolerant, have enhanced nutritional value, pest
resistant and reduce the destruction by disease causing
microorganims. This book is an essential foundation tool that is
available for researchers and students to reinforce the application
of Agrobacterium-mediated genetic transformation in soybean.
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