The purpose of Ribozyme Protocols is to provide a helpful
compilation of protocols that will be of use-^not only to those
with some experience of ribozymes-^but also to those wishing to use
ribozymes for the first time. Although it is usually impossible to
cover every aspect of a scientific field, I believe this book
approaches that ideal and should help all readers perform
meaningful experiments using ribozymes. To design ribozymes, one
must consider whether the target site will be accessible; this task
can be facilitated by using computer programs that pre dict the
folding of the target RNA. Such programs are detailed in Chapters 2
and 3. If the chosen target is an RNA virus that can mutate
rapidly, it makes sense to consider those parts of the genome that
are least likely to change during viral replication. An example of
how this can be done is described in Chapter 4. Although computer
analysis may be a useful starting point to select tar get sites,
there seems, at the moment, to be no guarantee that any particular
chosen site will be efficiently cleaved. Some workers have
deliberately bypassed this problem by using libraries of ribozyme
sequences and by select ing those that actually hybridize to and/or
cleave the target; these methods are described in Chapters 5 and 6.
General
Imprint: |
HumanaPress
|
Country of origin: |
United States |
Series: |
Methods in Molecular Biology, 74 |
Release date: |
August 2014 |
First published: |
1997 |
Editors: |
Philip C Turner
|
Dimensions: |
229 x 152 x 26mm (L x W x T) |
Format: |
Paperback
|
Pages: |
492 |
Edition: |
Softcover reprint of the original 1st ed. 1997 |
ISBN-13: |
978-1-4899-4285-2 |
Categories: |
Books >
Science & Mathematics >
Biology, life sciences >
Biochemistry >
General
|
LSN: |
1-4899-4285-8 |
Barcode: |
9781489942852 |
Is the information for this product incomplete, wrong or inappropriate?
Let us know about it.
Does this product have an incorrect or missing image?
Send us a new image.
Is this product missing categories?
Add more categories.
Review This Product
No reviews yet - be the first to create one!