|
Books > Science & Mathematics > Biology, life sciences > Biochemistry > Proteins
|
Buy Now
Difference Gel Electrophoresis (DIGE) - Methods and Protocols (Hardcover, 2012 ed.)
Loot Price: R3,128
Discovery Miles 31 280
|
|
|
Difference Gel Electrophoresis (DIGE) - Methods and Protocols (Hardcover, 2012 ed.)
Series: Methods in Molecular Biology, 854
Expected to ship within 10 - 15 working days
|
Protein analysis is increasingly becoming a cornerstone in
deciphering the molecular mechanisms of life. Proteomics, the
large-scale and high-sensitivity analysis of proteins, is already
pivotal to the new life sciences such as Systems Biology and
Systems Medicine. Proteomics, however, relies heavily on the past
and future advances of protein purification and analysis methods.
DIGE, being able to quantify proteins in their intact form, is one
of a few methods that can facilitate this type of analysis and
still provide the protein isoforms in an MS-compatible state for
further identification and characterization with high analytical
sensitivity. Differential Gel Electrophoresis: Methods and
Protocols introduces the concept of DIGE and its advantages in
quantitative protein analysis. It provides detailed protocols and
important notes on the practical aspects of DIGE with both generic
and specific applications in the various areas of Quantitative
Proteomics. Divided into four concise sections, this detailed
volume opens with the basics of DIGE, the technique and its
practical details with a focus on the planning of a DIGE experiment
and its data analysis. The next section introduces various DIGE
methods from those employed by scientists world-wide to more novel
methods, providing a glance at what is on the horizon in the DIGE
world. The volume closes with an overview of the wide range of DIGE
applications from Clinical Proteomics to Animal, Plant, and
Microbial Proteomics applications. Written in the highly successful
Methods in Molecular Biology (TM) series format, chapters contain
introductions to their respective topics, lists of the necessary
materials and reagents, step-by-step, readily reproducible
laboratory protocols, and notes on troubleshooting and avoiding
known pitfalls. Authoritative and accessible, Differential Gel
Electrophoresis: Methods and Protocols can be used by novices with
some background in biochemistry or molecular biology as well as by
experts in Proteomics who would like to deepen their understanding
of DIGE and its employment in many hyphenations and application
areas. With its many protocols, applications, and methodological
variants, it is also a unique reference for all who seek
fundamental details on the working principle of DIGE and ideas for
possible future uses of DIGE in novel analytical approaches.
General
Is the information for this product incomplete, wrong or inappropriate?
Let us know about it.
Does this product have an incorrect or missing image?
Send us a new image.
Is this product missing categories?
Add more categories.
Review This Product
No reviews yet - be the first to create one!
|
You might also like..
|
Welcome to Loot.co.za!
Sign in / Register |Wishlists & Gift Vouchers |Help | Advanced search
