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Books > Science & Mathematics > Biology, life sciences > Biochemistry
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Recombinant Protein Production in Yeast - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed. 2012)
Loot Price: R3,124
Discovery Miles 31 240
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Recombinant Protein Production in Yeast - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed. 2012)
Series: Methods in Molecular Biology, 866
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In the last few years, significant advances have been made in
understanding how a yeast cell responds to the stress of producing
a recombinant protein, and how this information can be used to
engineer improved host strains. The molecular biology of the
expression vector, through the choice of promoter, tag and codon
optimization of the target gene, is also a key determinant of a
high-yielding protein production experiment. Recombinant Protein
Production in Yeast: Methods and Protocols examines the process of
preparation of expression vectors, transformation to generate
high-yielding clones, optimization of experimental conditions to
maximize yields, scale-up to bioreactor formats and disruption of
yeast cells to enable the isolation of the recombinant protein
prior to purification. Written in the highly successful Methods in
Molecular Biology (TM) series format, chapters include
introductions to their respective topics, lists of the necessary
materials and reagents, step-by-step, readily reproducible
laboratory protocols, and key tips on troubleshooting and avoiding
known pitfalls. Authoritative and practical, Recombinant Protein
Production in Yeast: Methods and Protocols, seeks to aid scientists
in adopting yeast as a protein production host.
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