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Aspergillus niger as expression host for protein production (Paperback)
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Aspergillus niger as expression host for protein production (Paperback)
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Among the microorganisms, the filamentous fungi, as a group, have
contributed much to biotechnology particularly for the production
of enzymes and antibiotics and continue to have considerable
potential for further exploitation as producers of new products.
Few research groups have developed fungal expression systems using
specific host strains like, Aspergilus sojae, but their promoters
are already under IPR protection. Availability of expression vector
is a prerequisite in developing expression system and for that
strong promoters are needed. There, however, are no reports on the
catR promoter or its use in expression systems. Therefore, its
promoter is a legitimate target for cloning and exploitation. This
is the first report describing the identification of the regulatory
element of catR gene in Aspergillus niger. Clarifying the specific
induction or repression of the catR promoter provides the
possibility for utilization of this promoter in industry. Finally,
a finely controlled promoter can be used in studies of functional
characterization of essential genes.
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